Dr. Jia L. Song |
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Research Interests |
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The potential for forming a new organism begins at fertilization, when the sperm meets the egg. Across species from the worm to the human, development of the newly fertilized egg to a juvenile or an adult requires the careful regulation of cell growth, differentiation, and morphogenesis. Different cell types make different sets of proteins, even when their genomes are identical. What makes each cell type unique is a direct result of differential gene expressions mediated by transcription factors and signaling molecules in response to chemicals and proteins in the cell and the environment. Dysregulation of important genes involved in developmental decisions can lead to human diseases. Our research addresses one of the key questions in developmental biology: How are genes regulated during early development? |
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In 2006, Dr. Andrew Fire and Craig Mello were awarded the Nobel Prize in Physiology or Medicine for their work which discovered double stranded RNAs that trigger the suppression of gene activity. Their discovery revealed a novel mechanism of gene regulation mediated by microRNAs (miRNAs), which are small RNA molecules of 22 bases long that are expressed by all animals and plants. miRNAs are critical for all aspects of life, including the development of an organism and the physiological functions of cells and tissues. Because miRNAs are involved in regulating so many biological processes, their malfunction leads to many diseases such as cancer. With the increasing application of miRNAs in the treatment and diagnosis of various diseases, miRNAs have become an important tool in biological and medical research. With thousands of miRNAs identified, a pressing challenge is now to understand their specific biological functions by identifying their gene targets. |
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Current Projects: |
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• Another highly conserved miRNA that we focus on is miR-31 that has been examined mostly in the context of human cancer and more recently in bone formation. We use shotgun proteomics approach in identifying novel miR-31 functions as well as in discovering the regulatory mechanism of miR-31. Our results indicate that miR-31 regulates key transcription factors in the skeletogenesis gene regulatory network in the sea urchin and vertebrates. |
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• Highly conserved Wnt signaling pathways are critical for proper development. We hypothesize that the components of the Wnt signaling pathways are regulated by miRNAs. Using luciferase constructs and site-directed mutagenesis, we identified miRNA regulation on Disheveled and β-catenin, which encode key proteins central to Wnt signaling activation. Since miRNAs and components of the Wnt signaling pathways are known to be dysregulated in human diseases, results from our study may aid in therapeutics development resulting from aberrant miRNAs and Wnt signaling pathways.
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Summary of Center-Funded Research |
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